SEPARATION AND QUANTITATION OF HYDROXY AND EPOXY FATTY-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AN EVAPORATIVE LIGHT-SCATTERING DETECTOR

被引：27

作者：

GERARD, HC

MOREAU, RA

FETT, WF

OSMAN, SF

机构：

[1] Eastern Regional Research Center, U.S. Department of Agriculture, ARS, Philadelphia, 19118, PA

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1992年 / 69卷 / 04期

关键词：

DIHYDROXY FATTY ACIDS; EPOXY FATTY ACIDS; EVAPORATIVE LIGHT-SCATTERING DETECTOR; FATTY ACIDS; LIPIDS; NORMAL PHASE HPLC; RICINOLEIC ACID; TRIHYDROXY FATTY ACIDS;

D O I：

10.1007/BF02636054

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A new high-performance liquid chromatography technique with an evaporative light-scattering detector (ELSD) has been developed for the separation and quantitative analysis of hydroxy and epoxy fatty acids. This method employs a gradual binary gradient (hexane/isopropanol) and ELSD detection. The minimum limit of detection is about 1-mu-g and ratio of mass to signal is essentially linear in the range of 10 to 200-mu-g. This high-performance liquid chromatography (HPLC) technique is able to separate various positional isomers of monohydroxy and dihydroxy fatty acids and can also discriminate between monohydroxy, epoxy, epoxyhydroxy, dihydroxy and trihydroxy fatty acids.

引用

页码：301 / 304

页数：4

共 17 条

[1] VERNONIA-GALAMENSIS - A RICH SOURCE OF EPOXY ACID [J].