EVIDENCE INVITRO FOR AN ENZYMATIC-SYNTHESIS OF PHOSPHOCITRATE

被引：9

作者：

MORO, L ^{[1
]}

STAGNI, N ^{[1
]}

LUXICH, E ^{[1
]}

SALLIS, JD ^{[1
]}

DEBERNARD, B ^{[1
]}

机构：

[1] UNIV TASMANIA,DEPT BIOCHEM,HOBART,TAS 7001,AUSTRALIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0006-291X(90)91267-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biological synthesis of phosphocitrate is described from precursors, citrate and adenosinetriphosphate reacting in the presence or rat liver homogenate. Identity of the newly formed product was examined by enzymatic digestion of reactions mixtures, HPLC chromatography and 1H-NMR spectra. Authenticity of product was established by comparison to chemically synthesized phosphocitrate. Recognition of the existence of a biologically synthetic pathway adds credence to the known presence of phosphocitrate in mitochondria and a postulated role to control calcium phosphate deposition in that organelle. © 1990.

引用

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页码：251 / 258

页数：8

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