ANTEROGRADE AXONAL-TRANSPORT OF PEPTIDYLGLYCINE ALPHA-AMIDATING MONOOXYGENASE IN RAT SCIATIC-NERVES

被引：19

作者：

TOZAWA, K

ARAKAWA, E

CHIKUMA, T

OHHASHI, Y

YAJIMA, R

TAKEDA, K

SHINOZAKI, H

KATO, T

机构：

[1] YOKOHAMA CITY UNIV,GRAD SCH INTEGRATED SCI,MOLEC RECOGNIT LAB,22-2 SETO,KANAZAWA KU,YOKOHAMA 236,JAPAN

[2] TOKYO INST TECHNOL,DEPT LIFE CHEM,TOKYO 152,JAPAN

[3] DAINABOT CO LTD,RES INST,MATSUDO,JAPAN

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 55卷 / 03期

关键词：

Axonal transport; Cholecystokinin; Ligation; Peptidylglycine amidating enzyme; Peptidylglycine cr‐amidating monooxygenase; Sciatic nerve;

D O I：

10.1111/j.1471-4159.1990.tb04554.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: Axonal transport of peptidylglycine α‐amidating monooxygenase (PAM) activity was studied in rat sciatic nerves from 12 to 120 h after double ligations. The anterograde axonal transport increased and reached a plateau between 48 and 72 h and then decreased. The flow rate was 100 mm/day, and the molecular mass of the active entity was 70 kDa, which was determined by gel nitration. In contrast, there was no evidence for significant retrograde axonal transport. Anterograde axonal transport of immunoreactive cholecystokinin, a carboxy‐terminal‐amidated putative neuropeptide, was also found. These results suggest that PAM is transported by a rapid axonal flow and may play a role as a processing enzyme during transport or in the terminals of rat sciatic nerves. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：745 / 749

页数：5

共 18 条

[1] MECHANISM OF C-TERMINAL AMIDE FORMATION BY PITUITARY ENZYMES [J].