RAPID DIAGNOSIS OF TUBERCULOUS MENINGITIS BY POLYMERASE CHAIN-REACTION

被引:205
作者
SHANKAR, P
MANJUNATH, N
MOHAN, KK
PRASAD, K
BEHARI, M
SHRINIWAS
AHUJA, GK
机构
[1] ALL INDIA INST MED SCI,DEPT MICROBIOL,ANSARI NAGAR,NEW DELHI 110029,INDIA
[2] ALL INDIA INST MED SCI,DEPT NEUROL,NEW DELHI 110029,INDIA
关键词
D O I
10.1016/0140-6736(91)93328-7
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The polymerase chain reaction (PCR) in cerebrospinal fluid was compared with conventional bacteriology and an enzyme-linked immunosorbent assay (ELISA) for cerebrospinal fluid antibodies in the diagnosis of tuberculous meningitis (TBM). PCR was the most sensitive technique; it detected 15 (75%) of 20 cases of highly probably TBM (based on clinical features), 4 (57%) of 7 probable cases, and 3 (43%) of 7 possible cases. ELISA detected 11 (55%) of the highly probable cases and 2 each of the probable and possible cases. Culture was positive in only 4 of the highly probable cases. Among the controls (14 pyogenic meningitis, 3 aseptic meningitis, 34 other neurological disorders), 6 subjects tested early in the study (2 pyogenic meningitis, 4 other disorders) were PCR positive. Second DNA preparations from their stored cerebrospinal fluid samples were all PCR negative, suggesting that the false-positive results were due to cross-contamination. 18 PCR-positive TBM samples retested were all still PCR positive. The antibody ELISA was positive in 3 controls despite the use of a high cutoff value.
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页码:5 / 7
页数:3
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