SODIUM-GRADIENT DEPENDENCE OF PROLINE AND GLYCINE UPTAKE IN RAT RENAL BRUSH-BORDER MEMBRANE-VESICLES

被引：28

作者：

MCNAMARA, PD

PEPE, LM

SEGAL, S

机构：

[1] UNIV PENN,SCH MED,DEPT MED,PHILADELPHIA,PA 19174

[2] UNIV PENN,SCH MED,DEPT PEDIAT,PHILADELPHIA,PA 19174

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 556卷 / 01期

基金：

美国国家卫生研究院;

关键词：

(Rat kidney brush border vesicle); Amino acid transport; Ionic gradient dependence; Na[!sup]+[!/sup] affinity; Na[!sup]+[!/sup] dependence;

D O I：

10.1016/0005-2736(79)90427-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sodium-dependent entry of proline and glycine into rat renal brushborder membrane vesicles was examined. The high Km system for proline shows no sodium dependence. The low Km system for glycine entry is strictly dependent on a Na+ gradient but shows no evidence of the carrier system having any affinity for Na+. The low Km system for proline and high Km system for glycine transport appear to be shared. Both systems are stimulated by a Na+ gradient and appear to have an affinity for the Na+. The effect of decreasing the Na+ concentration in the ionic gradient is to alter the Km for amino acid entry and, at low Na+ concentrations, to inhibit the V for glycine entry. © 1979.

引用

页码：151 / 160

页数：10

共 14 条

[1]

Booth A G, 1974, Biochem J, V142, P575

[2]

CRANE RK, 1962, FED PROC, V21, P891

[3] PHENYLALANINE UPTAKE IN ISOLATED RENAL BRUSH-BORDER VESICLES [J].

EVERS, J ;

MURER, H ;

KINNE, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 426 (04) :598-615

[4]

FASS SJ, 1977, J BIOL CHEM, V252, P583

[5] FAMILIAL HYPERGLYCINURIA - NEW DEFECT IN RENAL TUBULAR TRANSPORT OF GLYCINE AND IMINO ACIDS [J].

GREENE, ML ;

LIETMAN, PS ;

ROSENBERG, LE ;

SEEGMILLER, JE .

AMERICAN JOURNAL OF MEDICINE, 1973, 54 (02) :265-271

[6]

HAMMERMAN MR, 1977, J BIOL CHEM, V252, P591

[7]

HILLMAN RE, 1969, J BIOL CHEM, V244, P4494

[8]

HILLMAN RE, 1968, J BIOL CHEM, V243, P5566

[9] METABOLISM AND UPTAKE OF L-PROLINE BY HUMAN KIDNEY-CORTEX [J].

HOLTZAPPLE, P ;

GENEL, M ;

REA, C ;

SEGAL, S .

PEDIATRIC RESEARCH, 1973, 7 (10) :818-825

[10] KINETICS OF L-HISTIDINE TRANSPORT IN PROXIMAL CONVOLUTION OF RAT NEPHRON STUDIED USING STATIONARY MICROPERFUSION TECHNIQUE [J].

LINGARD, J ;

RUMRICH, G ;

YOUNG, JA .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1973, 342 (01) :13-28

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