IMMOBILIZATION OF CATALASE BY POLYION COMPLEX

被引：9

作者：

KOHJIYA, S

MAEDA, K

YAMASHITA, S

机构：

[1] Department of Materials Science, Kyoto Institute of Technology, Kyoto, 606, Matsugasaki

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 1991年 / 43卷 / 12期

关键词：

D O I：

10.1002/app.1991.070431210

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Catalase was immobilized in a polyion complex from poly(sodium 2-acrylamide-2-methyl-1-propanesulfonate) and poly[2-(trimethylammonioethyl) methacrylate chloride]. Polyion complex formation between the two polyions was found to proceed stoichiometrically by the turbidimetric titration. Immobilization of catalase was achieved by conducting the complexation in the presence of the enzyme, which was established to be incorporated in the polyion complex networks. The fixed catalase showed higher thermal stability than the original enzyme. Furthermore, the stability and the activity of the fixed catalase were improved by annexing inert components such as albumin, histone, or lecithin at the time of polyion complex formation.

引用

页码：2219 / 2222

页数：4

共 12 条

[1]

AKABORI S, 1973, TANPAKUSHITSU KAGAKU

[2]

ATO T, 1973, BUNSEKI KAGAKU

[3] SEMIPERMEABLE MICROCAPSULES CONTAINING CATALASE FOR ENZYME REPLACEMENT IN ACATALASAEMIC MICE [J].

CHANG, TMS ;

POZNANSKY, MJ .

NATURE, 1968, 218 (5138) :243-+

[4]

CHIHATA I, 1975, IMMOBILIZED ENZYMES

[5]

FUKUI S, 1976, SEIKAGAKU, V48, P96

[6]

FUKUI S, 1976, SEIBUTSU YUKIKAGAKU, pCH5

[7] IMMOBILIZATION OF ALPHA-AMYLASE ONTO LATEX-PARTICLES [J].

HOSHINO, F ;

KAWAGUCHI, H ;

OHTSUKA, Y .

KOBUNSHI RONBUNSHU, 1985, 42 (05) :305-310

[8]

KOKUFUTA E, 1982, POLYM PREPR JPN, V31, P343

[9]

NAKAJIMA A, 1974, KAGAKU TO KOGYO, V27, P746

[10] WATER-INSOLUBLE DERIVATIVES OF ENZYMES ANTIGENS AND ANTIBODIES [J].

SILMAN, IH ;

KATCHALSKI, E .

ANNUAL REVIEW OF BIOCHEMISTRY, 1966, 35 :873-+

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