THE USE OF THE HUMAN NEUROBLASTOMA SH-SY5Y TO STUDY THE EFFECT OF 2ND-MESSENGERS ON NORADRENALINE RELEASE

被引:76
作者
VAUGHAN, PFT [1 ]
PEERS, C [1 ]
WALKER, JH [1 ]
机构
[1] UNIV LEEDS, DEPT BIOCHEM, LEEDS LS2 9JT, W YORKSHIRE, ENGLAND
来源
GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM | 1995年 / 26卷 / 06期
基金
英国惠康基金;
关键词
SH-SY5Y; NA RELEASE; CA2+ CHANNELS; PROTEIN KINASE;
D O I
10.1016/0306-3623(94)00312-B
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1. Recent data suggesting that the human neuroblastoma SH-SY5Y is a suitable cell line in which to study the effect of second messengers on NA release are discussed in the context of current views on exocytosis. 2. Release of NA is evoked by depolarization, as well as activation of muscarinic (M(3)) and bradykinin (B-2) receptors in SH-SY5Y cells which have not been differentiated by the addition of growth factors. 3. Evoked release is enhanced by activation of protein kinase C. 4. Activation of protein kinase C decreases the changes in intracellular calcium evoked by carbachol, bradykinin and 100 mM K+. 5. SH-SY5Y express N-type and L-type voltage sensitive Ca2+-channels. L-Type Ca2+-channels are coupled to NA release under conditions of weak depolarization. However with strong depolarization (100 mM K+) both L-type and N-type channels are involved. 6. Muscarinic- and neuropeptide Y receptors are coupled to the inhibition of Ca2+ channel activity.
引用
收藏
页码:1191 / 1201
页数:11
相关论文
共 74 条
[1]   MUSCARINIC RECEPTORS IN HUMAN SH-SY5Y NEUROBLASTOMA CELL-LINE - REGULATION BY PHORBOL ESTER AND RETINOIC ACID-INDUCED DIFFERENTIATION [J].
ADEM, A ;
MATTSSON, MEK ;
NORDBERG, A ;
PAHLMAN, S .
DEVELOPMENTAL BRAIN RESEARCH, 1987, 33 (02) :235-242
[2]  
AMMER H, 1994, J NEUROCHEM, V62, P1310
[3]   MODULATION OF VERTEBRATE NEURONAL CALCIUM CHANNELS BY TRANSMITTERS [J].
ANWYL, R .
BRAIN RESEARCH REVIEWS, 1991, 16 (03) :265-281
[4]   STUDIES ON THE MECHANISM OF [H-3] NORADRENALINE RELEASE FROM SH-SY5Y CELLS - THE ROLE OF CA2+ AND CYCLIC-AMP [J].
ATCHESON, R ;
LAMBERT, DG ;
HIRST, RA ;
ROWBOTHAM, DJ .
BRITISH JOURNAL OF PHARMACOLOGY, 1994, 111 (03) :787-792
[5]   REGULATION OF DOPAMINE RELEASE FROM PC12 PHEOCHROMOCYTOMA CELL-CULTURES DURING STIMULATION WITH ELEVATED POTASSIUM OR CARBACHOL [J].
BAIZER, L ;
WEINER, N .
JOURNAL OF NEUROCHEMISTRY, 1985, 44 (02) :495-501
[6]   INTRACELLULAR CA2+ BUFFERS DISRUPT MUSCARINIC SUPPRESSION OF CA2+ CURRENT AND M-CURRENT IN RAT SYMPATHETIC NEURONS [J].
BEECH, DJ ;
BERNHEIM, L ;
MATHIE, A ;
HILLE, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) :652-656
[7]   A DIFFUSIBLE 2ND MESSENGER MEDIATES ONE OF THE PATHWAYS COUPLING RECEPTORS TO CALCIUM CHANNELS IN RAT SYMPATHETIC NEURONS [J].
BERNHEIM, L ;
BEECH, DJ ;
HILLE, B .
NEURON, 1991, 6 (06) :859-867
[8]  
BLANCHARD S, 1993, T BIOCH SOC, V21, pS315
[9]   DISSECTION OF STAGES IN EXOCYTOSIS IN THE ADRENAL CHROMAFFIN CELL WITH USE OF TRIFLUOPERAZINE [J].
BURGOYNE, RD ;
GEISOW, MJ ;
BARRON, J .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1982, 216 (1202) :111-+
[10]   PRESYNAPTIC REGULATION OF NEUROTRANSMITTER RELEASE IN THE BRAIN - FACTS AND HYPOTHESIS [J].
CHESSELET, MF .
NEUROSCIENCE, 1984, 12 (02) :347-375