INVOLVEMENT OF FLAVIN COENZYME IN DIBENZOTHIOPHENE DEGRADING ENZYME-SYSTEM FROM RHODOCOCCUS-ERYTHROPOLIS D-1

被引：29

作者：

OHSHIRO, T ^{[1
]}

KANBAYASHI, Y ^{[1
]}

HINE, Y ^{[1
]}

IZUMI, Y ^{[1
]}

机构：

[1] TOTTORI UNIV, FAC ENGN, DEPT BIOTECHNOL, TOTTORI 680, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 07期

关键词：

D O I：

10.1271/bbb.59.1349

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the process of the purification of a dibenzothiophene (DBT)-degrading enzyme system from cell-free extracts of Rhodococcus erythropolis D-1, flavin coenzymes, FMN and PAD, were found to be involved in the enzymatic degradation of DBT in addition to NADH. Under these experimental conditions, the optimal concentrations of FMN and FAD were both 10 mu M and the activity was completely inhibited by adding 1 mM FMN or FAD. DBT was converted to DBT sulfone stoichiometrically and 2-hydroxybiphenyl formation was not observed when the reaction was done using the enzyme preparation purified by DEAE-Sepharose column chromatography.

引用

页码：1349 / 1351

页数：3

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