EFFECT OF LIPID SUPPLEMENTS ON THE PRODUCTION AND GLYCOSYLATION OF RECOMBINANT INTERFERON-GAMMA EXPRESSED IN CHO CELLS

被引：46

作者：

JENKINS, N ^{[1
]}

CASTRO, P ^{[1
]}

MENON, S ^{[1
]}

ISON, A ^{[1
]}

BULL, A ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,SERC,ADV CTR BIOCHEM ENGN,LONDON WC1E 7JE,ENGLAND

来源：

CYTOTECHNOLOGY | 1994年 / 15卷 / 1-3期

关键词：

GLYCOSYLATION; RECOMBINANT; INTERFERON; CHO CELLS; LIPIDS;

D O I：

10.1007/BF00762395

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of lipids on the glycosylation of recombinant human interferon-gamma expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-grycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-gamma deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-gamma glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.

引用

页码：209 / 215

页数：7

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