HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MICRO-DETERMINATION OF INDOPROFEN IN HUMAN-MILK

被引：7

作者：

LAKINGS, DB ^{[1
]}

LIZARRAGA, C ^{[1
]}

HAGGERTY, WJ ^{[1
]}

WILLIAMSON, MJ ^{[1
]}

机构：

[1] ADRIA LABS INC,COLUMBUS,OH 43216

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 1979年 / 68卷 / 09期

关键词：

Anti‐inflammatory agents—indoprofen; high‐performance liquid chromatographic analysis; human milk a Milk; human—analysis; indoprofen; high‐performance liquid chromatography; High‐performance liquid chromatography—analysis; human milk; Indoprofen—analysis;

D O I：

10.1002/jps.2600680914

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A previously reported high‐performance liquid chromatographic (HPLC) method for indoprofen determination in physiological fluids was modified and extended to provide quantitative data on drug concentrations in human milk samples at a low nanogram per milliliter level. The reversed‐phase HPLC technique was modified to give a better separation of the drug and milk components. To achieve the necessary cleanup for low level determination, the milk samples required protein precipitation, liquid‐liquid drug extraction, and concentration. Excellent indoprofen recovery was obtained with this technique; the average recovery from 20 milk samples spiked with various nanogram drug levels was 95%. The analytical technique showed excellent reproducibility; the calibration solutions over 15 days had a relative standard deviation of 3.2%. Results for indoprofen levels in milk and plasma samples from seven subjects who received either a single or multiple oral drug dose are presented. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company

引用

页码：1113 / 1116

页数：4

共 5 条

[1]

BUTTINON.A, 1973, ARZNEIMITTELFORSCH, V23, P1100

[2]

FUCCELLA LM, 1975, CLIN PHARMACOL THER, V17, P277

[3] FATE OF ANALGESIC AND ANTIINFLAMMATORY DRUG K-4277 AFTER ORAL-ADMINISTRATION TO MAN [J].