A GENOMIC CLONE ENCODING A CRYPTOPHYTE PHYCOERYTHRIN ALPHA-SUBUNIT - EVIDENCE FOR 3 ALPHA-SUBUNITS AND AN N-TERMINAL MEMBRANE TRANSIT SEQUENCE

被引：27

作者：

JENKINS, J

HILLER, RG

SPEIRS, J

GODOVAC-ZIMMERMANN, J

机构：

[1] MACQUARIE UNIV, SCH BIOL SCI, N RYDE, NSW 2109, AUSTRALIA

[2] CSIRO, DIV HORT, N RYDE, NSW 2113, AUSTRALIA

[3] AUSTRALIAN NATL UNIV, JOHN CURTIN SCH MED RES, CANBERRA, ACT 2600, AUSTRALIA

来源：

FEBS LETTERS | 1990年 / 273卷 / 1-2期

关键词：

Amino acid sequence; Chroomonas CS24; Cryptophyceae; Genomic clone; Phycoerythrin; Signal sequence; α-Subunit;

D O I：

10.1016/0014-5793(90)81082-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α1 and α2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing. © 1990.

引用

页码：191 / 194

页数：4

共 19 条

[1] LAMBDA-REPLACEMENT VECTORS CARRYING POLYLINKER SEQUENCES [J].