MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF CDNAS ENCODING HUMAN LONG-CHAIN ACYL-COA DEHYDROGENASE AND ASSIGNMENT OF THE LOCATION OF ITS GENE (ACADL) TO CHROMOSOME-2

被引:47
作者
INDO, Y [1 ]
YANGFENG, T [1 ]
GLASSBERG, R [1 ]
TANAKA, K [1 ]
机构
[1] YALE UNIV,SCH MED,DEPT HUMAN GENET,333 CEDAR ST,POB 3333,NEW HAVEN,CT 06510
关键词
D O I
10.1016/0888-7543(91)90068-P
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Long-chain acyl-CoA dehydrogenase (LCAD) catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids. We isolated and sequenced three cDNA clones encoding human LCAD precursor (p). The cDNAs encompass a 2217-base region including 5, 1290, and 922 bases in the 5′-noncoding, coding, and 3′-noncoding regions, respectively, and encodes the entire pLCAD of 430 amino acids (Mr: 47,656). The N-terminus of the mature human LCAD is currently unknown, but 30 (Mr 3221) and 400 amino acids (Mr: 44,435) of the sequence are considered to constitute the leader peptide and mature protein, respectively, in analogy to its rat counterpart. Human pLCAD cDNA shares 85.3 and 83.7% identical residues with rat pLCAD cDNA at the amino acid and nucleotide levels, respectively. At the amino acid level, human pLCAD shares 30.4 to 32.7% identical residues with three other human enzymes in the acyl-CoA dehydrogenase family, sharing 57 perfectly conserved residues among them. The human pLCAD gene is assigned to chromosome 2, bands q34-q35. © 1991.
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页码:609 / 620
页数:12
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