XYLAN STRUCTURE, MICROBIAL XYLANASES, AND THEIR MODE OF ACTION

被引:200
作者
BASTAWDE, KB
机构
[1] the Department of Biochemical Sciences, National Chemical Laboratory, Pune
关键词
BOND CLEAVAGE FREQUENCY; CHAINIA; ENDOXYLANASE; SUBSITE MAPPING;
D O I
10.1007/BF01198746
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Xylans, the major portion of the hemicellulose of plant cell walls and grasses, are.heteropolymers consisting principally of xylose and arabinose. Microbial xylanases with different multiplicities and properties are reported. Most studies on the mode of action of these xylanases have been carried out with fungi and there is very little information available on bacterial xylanases. Fungal xylanases have three or more substrate binding sites: for example Aspergillus niger, Ceratocytis paradoxa, Cryptococcus albidus and Chainia sp. endoxylanases have four to seven subsites with the catalytic site located at the centre of these sub-sites. The analysis of these sub-sites is either by kinetic or end-product analysis studies. Kinetic studies are used for exo-type enzymes while the end-product analysis studies are more convenient for endo-type enzymes. This review covers microbial xylanases with special emphasis on studies of sub-site mapping. The industrial applications of the microbial xylanases are also discussed.
引用
收藏
页码:353 / 368
页数:16
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  • [1] Ada G.A., Xylans: arabinoglucuronoxylan, arabinoxylan and xylan: purification using a copper complex and purification by fractional precipitation of acetales, Methods in Carbohydrate Chemistry, 5, pp. 170-175, (1965)
  • [2] Anand L., Vithayathil P.J., Production of extracellular xylanase by thermophilic fungus Humicola lanuginosa (Griffon and Maublane) Bunce, Indian Journal of Experimental Biology, 28, pp. 434-437, (1990)
  • [3] Angell S., Norris F.W., Hemicellulose V. Additional notes on the hemicelluloses of maize cobs, Biochem J, 30, pp. 2155-2165, (1936)
  • [4] Aspinall G.O., Structural Chemistry of hemicellulose, Advances in Carbohydrate Chemistry and Biochemistry, 14, pp. 429-468, (1959)
  • [5] Aspinall G.O., McKay J.E., The hemicellulose of European larch (Larix decidua), the constitution of a xylan, Journal of the Chemical Society, 1, pp. 1059-1066, (1959)
  • [6] Bastawde K.B., Studies on xylanase by Chainia sp., (1987)
  • [7] Bastawde K.B., Tabatabia L.B., Meagher M.M., Srinivasan M.C., Vartak H.G., Rele M.V., Reilly P.J., Catalytic properties and partial amino acid sequence of an actinomycete endoxylanase from Chainia sp, American Chemical Society Symposium Series, 460, pp. 417-425, (1991)
  • [8] Bernier R., Drignez H., Desrochers M., Molecular cloning of a Bacillus subtilis xylanase in Escherichia coli, Gene, 26, pp. 59-65, (1983)
  • [9] Bertrand J.-L., Morosoli R., Shareck F., Kluepfel D., Expression of the xylanase gene of Streptomyces lividans and production of the enzyme on natural substrates, Biotechnology and Bioengineering, 33, pp. 791-794, (1989)
  • [10] Bhalerao J., Patki A.H., Bhave M., Khurana I., Deobagkar D.N., Molecular cloning and expression of a xylanase gene from Cellulomonas sp. in to Escherichia coli, Applied Microbiology and Biotechnology, 34, pp. 71-76, (1990)