POLARIZATION SENSITIVE COHERENT ANTI-STOKES-RAMAN SCATTERING SPECTROSCOPY OF THE AMIDE I BAND OF PROTEINS IN SOLUTIONS

被引：40

作者：

CHIKISHEV, AY ^{[1
]}

LUCASSEN, GW ^{[1
]}

KOROTEEV, NI ^{[1
]}

OTTO, C ^{[1
]}

GREVE, J ^{[1
]}

机构：

[1] TWENTE UNIV TECHNOL,DEPT APPL PHYS,BIOPHYS TECHNOL GRP,7500 AE ENSCHEDE,NETHERLANDS

来源：

BIOPHYSICAL JOURNAL | 1992年 / 63卷 / 04期

关键词：

D O I：

10.1016/S0006-3495(92)81691-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Polarization sensitive coherent anti-Stokes Raman scattering (PCARS) spectroscopy is a fruitful technique to study Raman vibrations of diluted molecules under off-electron resonant conditions. We apply PCARS as a direct spectroscopic method to investigate the broad amide I band of proteins in heavy water. In spontaneous Raman spectroscopy, this band is not well resolved. We fit a number of spectra taken of each protein under different polarization conditions, with a single set of parameters. It then appears that some substructure is observed in the amide I band. From this substructure, we determine the percentage of alpha-helix, beta-sheet, and random coil for the proteins lysozyme, albumin, ribonuclease A, and alpha-chymotrypsin.

引用

页码：976 / 985

页数：10

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