DETERMINATION OF OCHRATOXIN-A IN URINE AND FECES OF SWINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：25

作者：

VALENTA, H

KUHN, I

ROHR, K

机构：

[1] Institut für Tierernährung der Bundesforschungsanstalt für Landwirtschaft, 3300 Braunschweig

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 613卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80145-T

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Sensitive methods for the determination of ochratoxin A in urine and faeces of swine are described. The samples were extracted with chloroform at pH <2, and the extracts were cleaned up by a combination of solid-phase extraction and liquid-liquid partition. High-performance liquid chromatography with fluorescence detection was used for detection and determination. The detection limits were 0.3 ng/ml for urine and 1.5 ng/g for faeces. Recoveries of ochratoxin A from spiked samples of urine and faeces were 93% and 60%, respectively. Because of the low detection limit and the fast and relatively easy performance, the method for the determination of ochratoxin A in urine proved suitable for the estimation of possible contamination of live animals.

引用

页码：295 / 302

页数：8

共 34 条

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[2]

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