PHENOTYPIC INVESTIGATION OF MONONUCLEAR PHAGOCYTES BY FLOW-CYTOMETRY

被引：38

作者：

STEINBACH, F ^{[1
]}

THIELE, B ^{[1
]}

机构：

[1] HUMBOLDT UNIV BERLIN, MED KLIN 3, BERLIN, GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 174卷 / 1-2期

关键词：

MONOCYTE; MACROPHAGE; DENDRITIC CELL; DIFFERENTIATION; FLOW CYTOMETRY; INTRACELLULAR ANTIGEN; (EQUINE);

D O I：

10.1016/0022-1759(94)90015-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid method for the phenotypic analysis of mononuclear phagocytes is described. Using flow cytometry, surface markers, as well as intracellular antigens and DNA content of peripheral blood monocytes, or differentiated monocytic cells could be analysed. Furthermore, the method has been of use in several species and is therefore not restricted to applications in humans. Different modes of data aquisition and analysis were compared and are discussed.

引用

页码：109 / 122

页数：14

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