INJECTION BIAS OF DNA FRAGMENTS IN CAPILLARY ELECTROPHORESIS WITH SIEVING

被引：19

作者：

KLEPARNIK, K

GARNER, M

BOCEK, P

机构：

[1] ACAD SCI CZECH REPUBL,INST ANALYT CHEM,CR-61142 BRNO,CZECH REPUBLIC

[2] NIH,PHYS & THEORET BIOL LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 698卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)00845-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The relative amounts of DNA fragments in a mixture injected into the capillary by electromigration or hydrodynamically by pressure were compared. Even if the electrophoretic mobilities of DNA fragments with different sizes are the same in a free solution in the sample vial, the size bias is brought about by the different mobilities in a sieving medium and by the electroosmosis. The experiments were performed in capillaries filled with a solution of liquified;agarose, a replaceable sieving medium. The experimental results were compared with a theoretical model.

引用

页码：375 / 383

页数：9

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