HOMOGENEITY AND SEQUENTIAL PERIODATE OXIDATION OF A GLYCOPEPTIDE FROM TAKA-AMYLASE A

The homogeneity of a glycopeptide obtained from Taka-amylase A [EC 3.2.1.1] was reinvestigated by column chromatography on Dowex 50×2 and high voltage paper electrophoresis. About 90 per cent of the glycopeptide was found to be homogeneous and to consist of one mole each of aspartic acid, serine and ammonia, 2 moles of N-acetylglucosamine and 6 moles of mannose.Sequential pcriodate oxidation of the purified homogeneous glycopeptide indicated that its carbohydrate sequence is Man-Man-GlcNAc-GlcNAc-NH. © 1969 BY THE JOURNAL OF BIOCHEMISTRY.