USE OF LACTATE-DEHYDROGENASE RELEASE TO ASSESS CHANGES IN CULTURE VIABILITY

被引：82

作者：

RACHER, AJ

LOOBY, D

GRIFFITHS, JB

机构：

[1] Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, SP4 0JG, Wiltshire, Porton Down

来源：

CYTOTECHNOLOGY | 1990年 / 3卷 / 03期

关键词：

culture viability; lactate dehydrogenase;

D O I：

10.1007/BF00365494

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study reports the use of lactate dehydrogenase release to monitor changes in culture viability in flask culture and fixed bed, porosphere bioreactor systems. Lactate dehydrogenase release shows good agreement with increase in non-viable cell numbers and decline in glucose utilisation in flask cultures. Studies with the immobilised system show that lactate dehydrogenase release can detect loss of viability which is not always indicated by a decrease in glucose utilisation. The data show that culture viability in a repeated-feed-and-harvest system is influenced markedly by both a) the medium change regime itself and b) the use of an immobilised bioreactor compared to a flask system for the same medium change regime. © 1990 Kluwer Academic Publishers.

引用

页码：301 / 307

页数：7

共 11 条

[1]

Clarke J.B., Spier R.E., Variation in the susceptibility of BHK populations and cloned cell lines to three strains of FMD virus, Arch. Virol., 86, pp. 1-9, (1980)

[2]

Cook J.A., Mitchell J.B., Viability measurements in mammalian cell systems, Anal. Biochem., 179, pp. 1-7, (1989)

[3]

Donnelly M., Scheffler I.E., Energy metabolism in respiration-deficient and wild type chinese hamster fibroblasts in culture, J. Cell Physiol., 89, pp. 39-52, (1976)

[4]

Griffiths J.B., Overview of cell culture systems and their scale-up, Animal Cell Biotechnol., 3, pp. 179-220, (1988)

[5]

Looby P., Griffiths J.B., Immobilisation of animal cells in fixed and fluidised porous glass sphere reactors, Advances in Animal Cell Biology and Technology for Bioprocesses, pp. 336-344, (1988)

[6]

Looby D., Griffiths J.B., Fixed bed porous glass sphere (porosphere) bioreactors for animal cells, Cytotechnology, 1, pp. 339-346, (1988)

[7]

Petersen J.F., McIntire L.V., Papoutsakis E.T., Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentration, J. Biotechnol., 7, pp. 229-246, (1988)

[8]

Reuveny S., Velez D., Macmillan J.D., Miller L., Factors affecting cell growth and monoclonal antibody production in stirred reactors, J. Immunol. Meth., 86, pp. 53-59, (1986)

[9]

Vassault A., Lactate dehydrogenase. UV-method with pyruvate and NADH, Methods of Enzymatic Analysis. III. Enzymes 1: Oxidoreductases, Transferases, pp. 118-126, (1983)

[10]

Wright J.P., Balfor A.H., Monoclonal antibodies to Toxoplasma gondii, Parasitol., 87, (1983)

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