RELATIONSHIP BETWEEN PHOSPHOINOSITIDE KINASE-ACTIVITIES AND PROTEIN TYROSINE PHOSPHORYLATION IN PLASMA-MEMBRANES FROM A431 CELLS

被引：47

作者：

PAYRASTRE, B

PLANTAVID, M

BRETON, M

CHAMBAZ, E

CHAP, H

机构：

[1] HOP PURPAN,INSERM,U326,F-31059 TOULOUSE,FRANCE

[2] CEN,INSERM,U244,BRCE,LBIO,F-38041 GRENOBLE,FRANCE

来源：

BIOCHEMICAL JOURNAL | 1990年 / 272卷 / 03期

关键词：

D O I：

10.1042/bj2720665

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of PtdIns(4) P and PtdIns(4,5)P2 by plasma-membrane preparations from A431 cells was selectively stimulated in a dose-dependent manner by epidermal growth factor (EGF) in the presence of Na3VO4. Na3 VO4 itself mimicked this effect, which was overcome after treatment by a specific phosphotyrosyl phosphatase isolated from A431 cells. PtdIns and PtdIns(4) P kinase activities were present in phosphotyrosyl-proteins isolated from EGF- and/or Na3VO4-stimulated A431 cells by immunoaffinity using an anti-phosphotyrosine antibody. These data suggest for the first time an EGF-dependent regulation of PtdIns 4-kinase and PtdIns(4) P 5-kinase activities by a mechanism involving a tyrosinephosphorylation process.

引用

页码：665 / 670

页数：6

共 31 条

[1] PDGF-DEPENDENT TYROSINE PHOSPHORYLATION STIMULATES PRODUCTION OF NOVEL POLYPHOSPHOINOSITIDES IN INTACT-CELLS [J].