DEVELOPMENT OF AN ANALYTICAL METHOD FOR CEPHAPIRIN AND ITS METABOLITE IN BOVINE-MILK AND SERUM BY LIQUID-CHROMATOGRAPHY WITH UV-VIS DETECTION AND CONFIRMATION BY THERMOSPRAY MASS-SPECTROMETRY

被引：24

作者：

TYCZKOWSKA, KL ^{[1
]}

VOYKSNER, RD ^{[1
]}

ARONSON, AL ^{[1
]}

机构：

[1] RES TRIANGLE INST,ANALYT & CHEM SCI,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS | 1991年 / 14卷 / 01期

关键词：

D O I：

10.1111/j.1365-2885.1991.tb00804.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Metabolites of the cephapirin beta-lactam antibiotic have not previously been reported in bovine milk. The principal metabolite was tentatatively identified as desacetylcephapirin by liquid chromatography with UV-VIS photodiode array (LC/UV-VIS PDA), and liquid-chromatography-mass-spectrometric (LC-MS) detection. Synthetic desacetylcephapirin was prepared by incubation of cephapirin in bovine milk and serum at 37-degrees-C. Also, a method for determining cephapirin in bovine milk and serum was developed. The detection limits for cephapirin and desacetylcephapirin were estimated to be 10 and 50-mu-g/kg, respectively, for LC/UV-VIS PDA, and 100 and 500-mu-g/kg for LC-MS.

引用

页码：51 / 60

页数：10

共 8 条

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