INTRACELLULAR BINDING OF ETHIDIUM STUDIED BY PHOTOAFFINITY-LABELING INVIVO

被引：12

作者：

FUKUNAGA, M ^{[1
]}

YIELDING, KL ^{[1
]}

机构：

[1] UNIV ALABAMA,MOLEC BIOL LAB,BIRMINGHAM,AL 35294

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 585卷 / 02期

关键词：

Ethidium binding; Photoaffinity labeling;

D O I：

10.1016/0304-4165(79)90029-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The azide analog of 14C-labeled ethidium bromide was mixed with yeast cells and when photolyzed by visible light, formed covalent complexes with all yeast cell organelles. The 14C counts were found in DNA, RNA and protein of yeast subcellular fractions, illustrating the complexity of binding of a drug which appears highly specific in its actions. © 1979.

引用

页码：293 / 299

页数：7

共 22 条

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[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 479 (01) : 98 - 104
[7] NUCLEAR GENE DOSAGE EFFECTS ON MITOCHONDRIAL MASS AND DNA
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[J]. JOURNAL OF CELL BIOLOGY, 1974, 61 (03) : 565 - 574
[8] HAWKING F, 1963, EXPT CHEMOTHERAPY, P129
[9] BYPASS BY PHOTOAFFINITY LABELING OF BLOCKED METABOLIC ACTIVATION OF ETHIDIUM - CONFIRMATION OF ROLE FOR COVALENT ETHIDIUM ATTACHMENT IN MITOCHONDRIAL MUTAGENESIS
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WHITE, WE
YIELDING, KL
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 66 (01) : 31 - 35
[10] HIXON SC, 1975, J MOL BIOL, V92, P319, DOI 10.1016/0022-2836(75)90231-4

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