ANALYSIS OF EXPRESSION AND EVOLUTIONARY RELATIONSHIPS OF PHOSPHOENOLPYRUVATE CARBOXYLASE GENES IN FLAVERIA-TRINERVIA (C-4) AND F-PRINGLEI (C-3)

被引:50
作者
HERMANS, J [1 ]
WESTHOFF, P [1 ]
机构
[1] UNIV DUSSELDORF,INST ENTWICKLUNGS & MOLEK BIOL PFLANZEN,W-4000 DUSSELDORF 1,GERMANY
来源
MOLECULAR & GENERAL GENETICS | 1990年 / 224卷 / 03期
关键词
FLAVERIA; C-3/C-4; PLANTS; PHOSPHOENOLPYRUVATE CARBOXYLASE; GENE FAMILY; EVOLUTION;
D O I
10.1007/BF00262441
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phosphoenolpyruvate carboxlylase (PEPCase) was shown to be encoded by a multigene family in various Flaveria species analysed. Several clones were isolated from genomic libraries of F. pringlei (C3 species) and F. trinervia (C4 species) and classified into four distinct groups according to their hybridization behaviour to a full-length cDNA clone encoding the PEPCase C4 isozyme of F. trinervia. A detailed cross-hybridization analysis demonstrated that the closest relative of most of the PEPCase genes isolated from F. trinervia and F. pringlei was not found in the same but in the other species. Northern analysis, using stringent conditions, allowed discrimination of class-specific PEPCase transcripts and revealed characteristic organ-specific expression patterns.
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页码:459 / 468
页数:10
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