AN IMPROVED TEST FOR AFRICANIZED HONEYBEE MITOCHONDRIAL-DNA

被引：99

作者：

CROZIER, YC

KOULIANOS, S

CROZIER, RH

机构：

[1] Department of Genetics and Human Variation, La Trobe University, Bundoora, 3083, V.

来源：

EXPERIENTIA | 1991年 / 47卷 / 09期

关键词：

AFRICANIZED HONEYBEE; APIS-MELLIFERA-SCUTELLATA; MITOCHONDRIAL DNA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; BGLII; POLYMERASE CHAIN REACTION;

D O I：

10.1007/BF01929894

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mitochondrial DNA derived from Apis mellifera scutellata, the ancestor of the Africanized bees of the New World, lacks a BglII restriction site found in other types of honeybee1,2. We present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. Digestion of the amplified product with BglII yields contrasting patterns between Africanized and other honeybees.

引用

页码：968 / 969

页数：2

共 12 条

[1]

BOYCE TM, 1989, GENETICS, V123, P825

[2] THE MITOCHONDRIAL-DNA MOLECULE OF DROSOPHILA-YAKUBA - NUCLEOTIDE-SEQUENCE, GENE ORGANIZATION, AND GENETIC-CODE [J].