DETERMINATION OF TRIMETHOPRIM IN BOVINE SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CONFIRMATION BY THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引:18
作者
NACHILOBE, P
BOISON, JO
CASSIDY, RM
FESSER, ACE
机构
[1] AGR CANADA,FOOD PROD & INSPECT BRANCH,HLTH ANIM LAB,SASKATOON S7N 2R3,SK,CANADA
[2] UNIV SASKATCHEWAN,DEPT CHEM,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 616卷 / 02期
关键词
D O I
10.1016/0378-4347(93)80392-H
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A high-performance liquid chromatographic (HPLC) method with a detection limit of 5 ng/ml was developed for the analysis of trimethoprim in bovine serum. Trimethoprim and the intemal standard, ormetoprim, under alkaline conditions, were first extracted into dichloromethane and then back-extracted into dilute sulphuric acid (0. 15 M) and cleaned-up on a C18 cartridge. Trimethoprim was quantified on a C18 column using a triethylammonium acetate-acetonitrile-methanol (16:3:1, v/v/v) mobile phase at a flow-rate of 1.5 ml/min, with ultraviolet detection at 225 nm. This method was used to verify the accuracy of test responses obtained with the Brilliant Black Reduction test, a rapid screening method, for trimethoprim levels in the serum of steers treated with Trivetrin. Confirmation of the presence of trimethoprim in the sample extract was obtained by thermospray HPLC-mass spectrometry.
引用
收藏
页码:243 / 252
页数:10
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