METHYLATION AT CPG SEQUENCES DOES NOT INFLUENCE HISTONE-H1 BINDING TO A NUCLEOSOME INCLUDING A XENOPUS-BOREALIS 5-S-RIBOSOMAL RNA GENE

被引：44

作者：

NIGHTINGALE, K ^{[1
]}

WOLFFE, AP ^{[1
]}

机构：

[1] NICHHD,MOLEC EMBRYOL LAB,BETHESDA,MD 20892

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 09期

关键词：

D O I：

10.1074/jbc.270.9.4197

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate that methylation of the 12 dinucleotide CpGs within a GC-rich DNA fragment containing a Xenopus borealis 5 S rRNA gene does not influence histone ill binding to naked or nucleosomal 5 S DNA. Thus a simple mechanism in which histone ill selectively associates with nucleosomes containing methylated CpG cannot explain the repressive effects of methylation on gene activity.

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页码：4197 / 4200

页数：4

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