EFFECTS OF BACILLUS-SUBTILIS SPORULATION REGULATORY PROTEIN SPOIIID ON TRANSCRIPTION BY SIGMA(K) RNA-POLYMERASE IN-VIVO AND IN-VITRO

SpoIIID is a sequence-specific, DNA-binding protein that activates or represses transcription of different genes by sigma(K) RNA polymerase in vitro. A Bacillus subtilis strain engineered to produce bath sigma(K) and SpoIIID during growth showed effects of SpoIIID on expression of sigma(K)-dependent genes that were consistent with the effects of a small amount of SpoIIID on transcription of these genes in vitro, indicating that the strain provides a simple, in vivo method to screen for effects of SpoIIID on transcription of sigma(K)-dependent genes.