DECREASED TRANSCRIPTION OF THE SODIUM-PHOSPHATE TRANSPORTER GENE IN THE HYPOPHOSPHATEMIC MOUSE

被引:22
作者
COLLINS, JF
SCHEVING, LA
GHISHAN, FK
机构
[1] VANDERBILT UNIV, SCH MED, DEPT PEDIAT, NASHVILLE, TN 37232 USA
[2] VANDERBILT UNIV, SCH MED, DEPT MOLEC PHYSIOL, NASHVILLE, TN 37232 USA
[3] VANDERBILT UNIV, SCH MED, DEPT BIOPHYS, NASHVILLE, TN 37232 USA
[4] VANDERBILT UNIV, SCH MED, DEPT PATHOL, NASHVILLE, TN 37232 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY | 1995年 / 269卷 / 03期
关键词
X-LINKED HYPOPHOSPHATEMIA; HYP MOUSE MODEL; TRANSCRIPTION RATE ANALYSIS;
D O I
10.1152/ajprenal.1995.269.3.F439
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Recently, it has been hypothesized that the proximal tubular Na+-P-i transporter may play a role in murine X-linked hypophosphatemic vitamin D-resistant rickets. In the present investigation, Western blot analysis of renal brush-border membrane proteins, utilizing polyclonal antisera raised against the mouse Na+-P-i transporter, revealed a predominant band at 87 kDa in normal and hypophosphatemic (Hyp) mice. The intensity of this band was reduced in the Hyp mouse by 4.5-fold (Hyp/normal = 0.22 +/- 0.04, n = 3, P < 0.05). Additionally, immunohistochemical analysis of kidney cortex in both mice localized the protein to the apical membrane of the proximal tubules. Relative transcription rates of the Na+-P-i transporter gene in the normal and Hyp mouse were then investigated. Nuclear run-on assays showed a 51 +/- 0.02% decreased rate of transcription of the Na+-P-i transporter gene in the Hyp mice (n = 3). Thus abnormal transcriptional control of this gene in the Hyp mouse likely plays a role in X-linked hypophosphatemia.
引用
收藏
页码:F439 / F448
页数:10
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