CHARACTERIZATION OF RNA SYNTHESIZED AT HIGH IONIC STRENGTH BY RAT LIVER AGGREGATE RNA POLYMERASE

被引：8

作者：

MANDEL, JL

CHAMBON, P

机构：

[1] Centre de Neurochimie, CNRS, Strasbourg

[2] Groupe de Recherche de l'INSERM, Faculté de Médecine, Strasbourg

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 35卷 / 06期

关键词：

D O I：

10.1016/0006-291X(69)90704-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNAs synthesized in vitro at high ionic strength by rat liver nuclear aggregate RNA polymerase were characterized by DNA-RNA hybridization. Results indicate that all the RNA species which were synthesized in vitro and which hybridized with rat liver DNA were also present in vivo in rat liver nucleus. Only a fraction of these in vitro synthesized RNAs were found in vivo both in nucleus and cytoplasm. These results suggest that RNA synthesis catalyzed in vitro at high ionic strength by the aggregate RNA polymerase retains most of the specificity of RNA synthesis in vivo. © 1969.

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页码：868 / &

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