VACUOLAR PROTON-PUMPING PYROPHOSPHATASE IN BETA-VULGARIS SHOWS VECTORIAL ACTIVATION BY POTASSIUM

被引：38

作者：

DAVIES, JM

REA, PA

SANDERS, D

机构：

[1] UNIV YORK,DEPT BIOL,YORK YO1 5DD,N YORKSHIRE,ENGLAND

[2] UNIV PENN,DEPT BIOL,INST PLANT SCI,PHILADELPHIA,PA 19104

来源：

FEBS LETTERS | 1991年 / 278卷 / 01期

关键词：

H+-PYROPHOSPHATASE; POTASSIUM; TONOPLAST; PATCH CLAMP; BETA-VULGARIS;

D O I：

10.1016/0014-5793(91)80085-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous work with membrane vesicles has demonstrated an absolute dependence on K+ for proton translocation by the inorganic pyrophosphatase (H+ -PPase: EC 3.6.1.1) from the vacuolar membrane (tonoplast) of higher plants. Using intact vacuoles from sugar beet (Beta vulgaris) storage tissue, we have monitored PP(i)-dependent currents by patch clamp in 'whole vacuole' mode. Serial K+ substitutions were made at both tonoplast faces. The results show that K+ activation occurs only at the cytosolic face.

引用

页码：66 / 68

页数：3

共 16 条

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