VISCOELASTIC BEHAVIOR OF MAMMALIAN DNA

被引：19

作者：

CHASE, ES ^{[1
]}

SHAFER, RH ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH MED,BRAIN TUMOR RES CTR,SAN FRANCISCO,CA 94143

来源：

BIOPHYSICAL JOURNAL | 1979年 / 28卷 / 01期

关键词：

D O I：

10.1016/S0006-3495(79)85161-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The viscoelastic behavior of rat 9L cellular DNA was studied as a function of the detergent used for lysis, the pH and duration of lysis, and gamma ray dose. For nondenaturing lysis conditions, a model of the DNA was proposed to account for the effects of these agents on the viscoelastic retardation time. It was concluded that these agents affect the hydrodynamic radius of the DNA rather than its molecular weight. For denaturing lysis conditions, molecular weights calculated from the relaxation time were consistent with those calculated from alkaline sucrose sedimentation profiles. © 1979, The Biophysical Society. All rights reserved.

引用

页码：93 / 105

页数：13