A SIMPLE METHOD OF CONCENTRATING MONOCLONAL-ANTIBODIES FROM CULTURE SUPERNATANT BY ULTRAFILTRATION

被引：6

作者：

SAHA, K

CASE, R

WONG, PKY

机构：

[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT CARCINOGENESIS,DIV SCI PK RES,POB 389,SMITHVILLE,TX 78957

[2] UNIV TEXAS,EL PASO,TX 79968

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 151卷 / 1-2期

关键词：

ULTRAFILTRATION; CULTURE SUPERNATANT; MONOCLONAL ANTIBODY;

D O I：

10.1016/0022-1759(92)90131-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

How to obtain concentrated monoclonal antibodies from culture supernatant is of great importance to many scientists today. In this report, a simple, single-step technique is described to concentrate monoclonal antibodies directly from hybridoma-culture supernatant. The contaminants can be removed from the supernatant by using an ultrafiltration unit which allows macromolecules of less than 100,000 MW to pass through leaving behind monoclonal antibodies which is purified and concentrated more than 25-fold.

引用

页码：307 / 308

页数：2

共 7 条

[1] MEMBRANE ULTRAFILTRATION - DIAFILTRATION TECHNIQUE AND ITS APPLICATION TO MICROSOLUTE EXCHANGE AND BINDING PHENOMENA [J].