DISSOLVED FREE AMINO-ACIDS, COMBINED AMINO-ACIDS, AND DNA AS SOURCES OF CARBON AND NITROGEN TO MARINE-BACTERIA

被引:145
作者
JORGENSEN, NOG
KROER, N
COFFIN, RB
YANG, XH
LEE, C
机构
[1] NATL ENVIRONM RES INST, DEPT MARINE ECOL & MICROBIOL, FREDERIKSBORGVEJ 399, DK-4000 ROSKILDE, DENMARK
[2] ROYAL VET & AGR UNIV, DEPT ECOL & MOLEC BIOL, MICROBIOL SECT, DK-1958 FREDERIKSBERG C, DENMARK
[3] US EPA, GULF BREEZE ENVIRONM RES LAB, SABINE ISL, FL 32561 USA
[4] SUNY, MARINE SCI RES CTR, STONY BROOK, NY 11794 USA
关键词
D O I
10.3354/meps098135
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Utilization of naturally-occurring dissolved free and combined amino acids (DFAA and DCAA) and dissolved DNA (D-DNA) was studied in batch cultures of bacteria from 2 shallow marine environments, Santa Rosa Sound (SRS), Florida, USA, and Flax Pond (FP), Long Island, New York, USA. In addition to control experiments, cultures were enriched with various sources of C and N. In the SRS cultures, net C assimilation of DCAA, DFAA and D-DNA made up from 42 to 60 % of net bacterial C production. The average, relative incorporation of C from DCAA, DFAA and D-DNA was 0.13, 0.77 and 0.10, respectively. The corresponding values of N were 0.10, 0.81 and 0.09. Addition of NH4+, DFAA, or both, increased the incorporation of DCAA, DFAA and D-DNA. Incorporation of N from DCAA, DFAA and D-DNA equaled 111 to 219 % of the bacterial N production. Including incorporation of NH4+ and release of NO3-, calculated net bacterial N incorporation made up 48 and 290 % of bacterial N production. In the FP experiment, net assimilation of DCAA, DFAA and D-DNA equaled 66 to 91 % (average 77 %) of net bacterial C production. Largely all C incorporation was due to assimilation of DFAA. Enrichment of the cultures with NH4+, glucose, methylamines, and high-molecular-weight dissolved organic matter all increased bacterial production. Net N incorporation of DCAA, DFAA and D-DNA corresponded to between 100 and 180 % of net bacterial N production. In 3 cultures DFAA were the dominant (> 50 %) bacterial N source. In the other cultures, DFAA and NH4+ were similar in importance as an N source, and together they accounted for 80 to 99 % of bacterial N incorporation. Including uptake of NH4+ and uptake or release of NO3- in the N budgets, bacterial N incorporation equaled 155 to 413 % of bacterial N production. The reason for this apparently excessive N uptake in both sets of cultures may be the release of organic nitrogen compounds.
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页码:135 / 148
页数:14
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