ROLE OF CALCIUM-ION IN INDUCTION OF APOPTOSIS BY ETOPOSIDE IN HUMAN LEUKEMIA HL-60 CELLS

被引：37

作者：

YOSHIDA, A ^{[1
]}

UEDA, T ^{[1
]}

TAKAUJI, R ^{[1
]}

LIU, YP ^{[1
]}

FUKUSHIMA, T ^{[1
]}

INUZUKA, M ^{[1
]}

NAKAMURA, T ^{[1
]}

机构：

[1] FUKUI MED SCH,DEPT BIOCHEM 1,FUKUI 91011,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.2338

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The epipodophyllotoxin derivative etoposide, an inhibitor of DNA topoisomerase II, has been found to induce internucleosomal DNA fragmentation, characteristic of apoptosis. In the present study, we examined the effect of Ca2+ depletion on VP-16-induced endonucleolytic DNA cleavage in HL-60 cells. VP-16-induced internucleosomal DNA fragmentation could not be blocked by the extracellular Ca2+ chelator EGTA. However, an intracellular Ca2+ chelator BAPTA-AM, which was added after treatment with VP-l6, abolished both internucleosomal DNA fragmentation and the morphologic features of apoptosis. No significant increase of intracellular Ca2+ was found after VP-16 treatment. We demonstrate for the first time that preexisting intracellular Ca2+ plays an essential role in induction of apoptosis by VP-16 in HL-60 cells. © 1993 Academic Press. All rights reserved.

引用

页码：927 / 934

页数：8

共 17 条

[1]

BARRY MA, 1993, CANCER RES, V53, P2349

[2] IDENTIFICATION OF DEOXYRIBONUCLEASE-II AS AN ENDONUCLEASE INVOLVED IN APOPTOSIS [J].