MEMBRANE-PERMEABILIZING ACTIVITIES OF BACILLUS-THURINGIENSIS COLEOPTERAN-ACTIVE TOXIN CRYIIIB2 AND CRYIIIB2 DOMAIN-I PEPTIDE

被引:68
作者
VONTERSCH, MA [1 ]
SLATIN, SL [1 ]
KULESZA, CA [1 ]
ENGLISH, LH [1 ]
机构
[1] ALBERT EINSTEIN COLL MED, DEPT PHYSIOL & BIOPHYS, NEW YORK, NY 10461 USA
关键词
D O I
10.1128/AEM.60.10.3711-3717.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacillus thuringiensis toxin CryIIIB2 exhibits activity against two agriculturally important pests, the Colorado potato beetle, Leptinotarsa decemlineata, and the Southern corn rootworm, Diabrotica undecimpunctata. CryIIIB2 shows significant structural similarity to Colorado potato beetle-active toxin CryIIIA, whose crystal structure has been determined elsewhere [J. Li, J. Carrol, and D. J. Ellar, Nature (London) 353:815-821, 1991]. A clone limited to the putative 7-alpha-helical bundle domain I peptide of CryIIIB2 was constructed by PCR. The truncated protein was expressed at high levels in Escherichia coli. Domain I peptide was isolated and compared with native CryIIIB2 toxin in promoting ion efflux from synthetic phospholipid vesicles and formation of ion channels in black lipid membranes. The results showed that CryIIIB2 domain I peptide is sufficient for ion channel formation and promotes ion efflux. Both native CryIIIB2 toxin and domain I peptide were inefficient channel-forming proteins that produced noisy ion channels of various conductance states. In ion efflux assays, native toxin promoted greater ion efflux from synthetic vesicles than did the truncated peptide.
引用
收藏
页码:3711 / 3717
页数:7
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