STUDIES OF THE ORIENTATION OF THE MITOCHONDRIAL REDOX CARRIERS .3. ORIENTATION OF THE GX AND GY AXES OF THE HEMES OF CYTOCHROME-OXIDASE WITH RESPECT TO THE PLANE OF THE MEMBRANE IN ORIENTED MEMBRANE MULTILAYERS

被引:22
作者
ERECINSKA, M
WILSON, DF
BLASIE, JK
机构
[1] Department of Biochemistry and Biophysics, University of Pennsylvania, Medical School, Philadelphia
关键词
(Multilayer; Mitochondrial membrane); Cytochrome oxidase; ESR; Heme orientation; Redox carrier;
D O I
10.1016/0005-2728(79)90212-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The EPR absorption properties of the hemes of cytochrome oxidase and their liganded derivatives were examined in oriented multilayers from isolated oxidase, mitochondrial membranes and membrane fragments of a bacterium, Paracoccus denitrificans. The hemes of the oxidase in all the systems investigated were oriented normal to the plane of the multilayers. The directions of the g signals corresponding to the gx and gy axes of the g tensor were found to be different in low-spin ferric heme in fully oxidized oxidase and in half-reduced liganded oxidase. It is suggested that this different orientation of gx and gy in fully oxidized oxidase and half-reduced liganded oxidase arises because the respective EPR signals belong to two different hemes, those of cytochrome a and a3. © 1979.
引用
收藏
页码:352 / 364
页数:13
相关论文
共 25 条
[1]   STRUCTURE OF A CYTOCHROME OXIDASE-LIPID MODEL MEMBRANE [J].
BLASIE, JK ;
ERECINSKA, M ;
SAMUELS, S ;
LEIGH, JS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 501 (01) :33-52
[2]   ORIENTATION OF A HEME OF CYTOCHROME-C OXIDASE IN SUBMITOCHONDRIAL PARTICLES [J].
BLUM, H ;
HARMON, HJ ;
LEIGH, JS ;
SALERNO, JC ;
CHANCE, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 502 (01) :1-10
[3]   CYTOCHROME-C OXIDASE - SYNOPSIS [J].
ERECINSKA, M ;
WILSON, DF .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1978, 188 (01) :1-14
[4]   STUDIES ON ORIENTATIONS OF MITOCHONDRIAL REDOX CARRIERS .1. ORIENTATION OF HEMES OF CYTOCHROME-C OXIDASE WITH RESPECT TO PLANE OF A CYTOCHROME OXIDASE-LIPID MODEL MEMBRANE [J].
ERECINSKA, M ;
WILSON, DF ;
BLASIE, JK .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 501 (01) :53-62
[5]   ORIENTATION OF HEMES OF CYTOCHROME-C OXIDASE AND CYTOCHROME-C IN MITOCHONDRIA [J].
ERECINSKA, M ;
BLASIE, JK ;
WILSON, DF .
FEBS LETTERS, 1977, 76 (02) :235-239
[6]   STUDIES ON ORIENTATIONS OF MITOCHONDRIAL REDOX CARRIERS .2. ORIENTATION OF MITOCHONDRIAL CHROMOPHORES WITH RESPECT TO PLANE OF MEMBRANE IN HYDRATED, ORIENTED MITOCHONDRIAL MULTILAYERS [J].
ERECINSKA, M ;
WILSON, DF ;
BLASIE, JK .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 501 (01) :63-71
[7]   REGULATION OF ENERGY-METABOLISM - EVIDENCE AGAINST A PRIMARY ROLE OF ADENINE-NUCLEOTIDE TRANSLOCASE [J].
ERECINSKA, M ;
KULA, T ;
WILSON, DF .
FEBS LETTERS, 1978, 87 (01) :139-144
[8]   B CYTOCHROMES IN SUCCINATE-CYTOCHROME-C REDUCTASE FROM PIGEON BREAST MITOCHONDRIA [J].
ERECINSKA, M ;
OSHINO, R ;
OSHINO, N ;
CHANCE, B .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 157 (02) :431-445
[9]  
GIBSON J F, 1957, Nature, V180, P29, DOI 10.1038/180029a0
[10]   ELECTRON RESONANCE STUDIES OF HAEMOGLOBIN DERIVATIVES .3. LINE-WIDTH AND G-VALUE MEASUREMENTS OF ACID-MET MYOGLOBIN AND OF MET MYOGLOBIN AZIDE DERIVATIVES [J].
HELCKE, GA ;
INGRAM, DJE ;
SLADE, EF .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1968, 169 (1016) :275-+