CHARACTERIZATION, CLONING AND HOST-PROTECTIVE ACTIVITY OF A 30-KILODALTON GLYCOPROTEIN SECRETED BY THE PARASITIC STAGES OF TRICHOSTRONGYLUS-COLUBRIFORMIS

被引：38

作者：

SAVIN, KW

DOPHEIDE, TAA

FRENKEL, MJ

WAGLAND, BM

GRANT, WN

WARD, CW

机构：

[1] CSIRO,DIV ANIM HLTH,SYDNEY,NSW,AUSTRALIA

[2] CSIRO,DIV ANIM HLTH,ADELAIDE,SA 5001,AUSTRALIA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1990年 / 41卷 / 02期

关键词：

Expression; Gene; Glycoprotein; Nematode; Secretion; Trichostrongylus;

D O I：

10.1016/0166-6851(90)90179-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The helminth Trichostrongylus colubriformis is a parasitic nematode infecting the small intestine of sheep. We report the isolation and characterization of a 30-kDa glycoprotein capable of partially protecting guinea-pigs against the parasite. This glycoprotein is secreted by the L4 and adult parasitic stages of the worm. The sequence of three separate cDNA clones predicts the polypeptide to be about 15 kDa, with four N-linked carbohydrate chains and an internal disulphide bond. The clones also indicate the existence of sequence variability in this antigen. Limited sequence homology to a porcine intestinal peptide suggests an influence on host gut physiology. © 1990.

引用

页码：167 / 176

页数：10

共 39 条

[1]

ADAMS DB, 1984, REV RURAL SCI, V6, P67

[2] GASTRIC DYSFUNCTION IN SHEEP INFECTED WITH TRICHOSTRONGYLUS-COLUBRIFORMIS, A NEMATODE INHABITING THE SMALL-INTESTINE [J].