ABSORPTION OF PROTAMINE-INSULIN IN DIABETIC-PATIENTS .1. PREPARATION AND CHARACTERIZATION OF PROTAMINE-INSULIN-I-125

被引：17

作者：

HANSEN, B ^{[1
]}

LINDE, S ^{[1
]}

KOLENDORF, K ^{[1
]}

JENSEN, F ^{[1
]}

机构：

[1] NORDISK INSULINLAB,GENTOFTE,DENMARK

来源：

HORMONE AND METABOLIC RESEARCH | 1979年 / 11卷 / 02期

关键词：

D O I：

10.1055/s-0028-1092685

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Protamine-125I-insulin with low specific radioactivity was prepared using 125,127I-insulin, 0.2 I/mole. The preparations were characterized by disc electrophoresis, isoelectric focusing and analytical gel chromatography in order to evaluate the suitability of 125I-insulin as marker for insulin in protamine-insulin. The stability of the preparations was followed up to 90 days at 4°C. The biological and immunological activity was determined in mice, isolated rat fat cells and by radioimmunoassay. It was concluded that both from a chemical and a biological point of view, the protamine-125I-insulin is as a satisfactory preparation that might be used in absorption studies.

引用

页码：85 / 90

页数：6

共 16 条

[1]

BINDER C, 1969, THESIS MUNKSGAARD

[2]

BINDER C, 1973, BRIT PHARMACOPOEA, pA109

[3] STORAGE TELEMETRIC SYSTEM FOR DETECTION OF RADIONUCLIDE TRACERS IN HUMANS [J].