INTERACTION OF MITOCHONDRIAL CREATINE-KINASE WITH MODEL MEMBRANES - A MONOLAYER STUDY

被引：47

作者：

ROJO, M

HOVIUS, R

DEMEL, R

WALLIMANN, T

EPPENBERGER, HM

NICOLAY, K

机构：

[1] SWISS FED INST TECHNOL,INST CELL BIOL,CH-8093 ZURICH,SWITZERLAND

[2] STATE UNIV UTRECHT,CTR BIOMEMBRANES & LIPID ENZYMOL,3584 CH UTRECHT,NETHERLANDS

[3] STATE UNIV UTRECHT,INST MOLEC BIOL & MED BIOTECHNOL,3584 CH UTRECHT,NETHERLANDS

来源：

FEBS LETTERS | 1991年 / 281卷 / 1-2期

关键词：

CREATINE KINASE; MODEL MEMBRANE; PROTEIN-LIPID INTERACTION; MITOCHONDRION;

D O I：

10.1016/0014-5793(91)80374-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of mitochrondrial creatine kinase (Mi-CK; EC 2.7.3.2) with phospholipid monolayers and spread mitochondrial membranes at the air/water interface has been investigated. It appeared that Mi-CK penetrated into these monolayers as evidenced by an increase in surface pressure upon incorporation of Mi-CK. The increase in surface pressure was dependent on (1) the amount and (2) the oligomeric form of Mi-CK in the subphase, as well as on (3) the initial surface pressure and (4) the phospholipid composition of the monolayer. In this experimental system Mi-CK was able to interact equally well with both inner and outer mitochondrial membranes.

引用

页码：123 / 129

页数：7

共 38 条

[1] FURTHER CHARACTERIZATION OF CONTACT SITES FROM MITOCHONDRIA OF DIFFERENT TISSUES - TOPOLOGY OF PERIPHERAL KINASES [J].