MUTATION INDUCED BY DEOXYXANTHOSINE IN CODON-12 OF A SYNTHETIC C-HA-RAS GENE

被引：42

作者：

KAMIYA, H ^{[1
]}

SHIMIZU, M ^{[1
]}

SUZUKI, M ^{[1
]}

INOUE, H ^{[1
]}

OHTSUKA, E ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA 12,NISHI 6,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

NUCLEOSIDES & NUCLEOTIDES | 1992年 / 11卷 / 2-4期

关键词：

D O I：

10.1080/07328319208021700

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To evaluate the base-pairing properties and mutagenicity of deoxyxanthosine in DNA, the modified base was incorporated into a synthetic c-Ha-ras gene and a DNA transfection experiment was done. The ras gene containing deoxyxanthosine showed very high focus-forming activity. Analysis of the genes from transformants showed almost exclusively a transition of G to A. These results indicate that dTMP was preferentially incorporated at the site opposite to deoxyxanthosine, and deoxyxanthosine can induce G to A transitions in mammalian cells.

引用

页码：247 / 260

页数：14

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