REGULATION OF RAT-KIDNEY CORTEX FRUCTOSE-1,6-BISPHOSPHATASE ACTIVITY .1. EFFECTS OF FRUCTOSE-2,6-BISPHOSPHATE AND DIVALENT-CATIONS

被引：11

作者：

SOLA, MM ^{[1
]}

OLIVER, FJ ^{[1
]}

SALTO, R ^{[1
]}

GUTIERREZ, M ^{[1
]}

VARGAS, AM ^{[1
]}

机构：

[1] UNIV GRANADA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,GRANADA,SPAIN

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 12期

关键词：

D O I：

10.1016/0020-711X(88)90332-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. The native rat-kidney cortex Fructose-1,6-BPase is differentially regulated by Mg2+ and Mn2+. 2. Mg2+ binding to the enzyme is hyperbolic and large concentrations of the cation are non-inhibitory. 3. Mn2+ produces a 10-fold rise in V-max higher than Mg2+. [Mg-2+](0.5) is much larger than [Mg2+](0.5). At elevated [Mn2+] inhibition is observed. 4. Mg2+ and Mn2+ produce antagonistic effects on the inhibition of the enzyme by high substrate. 5. Fru-2,6-P-2, inhibits the enzyme by rising the S-0.5 and favouring a sigmoidal kinetics. 6. The inhibition by Fru-2,6-P-2 is released by Mg2+ and more powerfully by Mn2+ increasing the I-0.5.

引用

页码：1963 / 1968

页数：6

共 37 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

CARLSON CW, 1975, J BIOL CHEM, V250, P4996

[3] ON THE MECHANISM OF INHIBITION OF NEUTRAL LIVER FRUCTOSE 1,6-BISPHOSPHATASE BY FRUCTOSE 2,6-BISPHOSPHATE [J].

FRANCOIS, J ;

VANSCHAFTINGEN, E ;

HERS, HG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 134 (02) :269-273

[4] KINETIC DIFFERENCES BETWEEN 2 INTERCONVERTIBLE FORMS OF FRUCTOSE-1,6-BISPHOSPHATASE FROM SACCHAROMYCES-CEREVISIAE [J].

GANCEDO, JM ;

MAZON, MJ ;

GANCEDO, C .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1982, 218 (02) :478-482

[5] GLUCONEOGENESIS AND RELATED ASPECTS OF GLYCOLYSIS [J].

HERS, HG ;

HUE, L .

ANNUAL REVIEW OF BIOCHEMISTRY, 1983, 52 :617-653

[6]

Hue L, 1981, Adv Enzymol Relat Areas Mol Biol, V52, P247

[7]

KITAJIMA S, 1983, J BIOL CHEM, V258, P7352

[8]

LIU F, 1988, J BIOL CHEM, V263, P10035

[9] THE SITE OF SUBSTRATE AND FRUCTOSE 2,6-BISPHOSPHATE BINDING TO RABBIT LIVER FRUCTOSE-1,6-BISPHOSPHATASE [J].

LIU, F ;

ROY, M ;

FROMM, HJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (02) :689-695

[10] PURIFICATION AND CHARACTERIZATION OF FRUCTOSE-1,6-BISPHOSPHATASE FROM BOVINE BRAIN [J].

LIU, F ;

FROMM, HJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 260 (02) :609-615

← 1 2 3 4 →