APICAL PLASMA-MEMBRANE VESICLES FORMED FROM ORGAN DONOR COLON DEMONSTRATE NA+ AND H+ CONDUCTANCES AND NA+/H+ EXCHANGE

被引：23

作者：

HARIG, JM

DUDEJA, PK

KNAUP, SM

SHOSHARA, J

RAMASWAMY, K

BRASITUS, TA

机构：

[1] VET ADM MED CTR,WASHINGTON,DC 20422

[2] MED COLL WISCONSIN,MILWAUKEE,WI 53226

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92042-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apical plasma membrane vesicles were prepared from human organ donor colon mucosal scrapings. These vesicles were enriched 10-fold in cysteine-sensitive alkaline phosphatase activity compared to starting homogenates, and showed minimal contamination of microsomal, mitochondrial or basolateral membranes. Transport studies using [22Na] uptake into proximal colonic vesicles demonstrated Na+ and H+ conductances, Na+ H+ exchange and amiloride inhibition of Na+ uptake. The isolation of these apical vesicles will permit detailed study of human colonic transport processes. © 1990.

引用

页码：438 / 443

页数：6

共 19 条

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