ALTERATIONS OF ONCOGENES IN METASTATIC TUMORS OF HUMAN GASTRIC CARCINOMAS

被引:66
作者
TSUJINO, T
YOSHIDA, K
NAKAYAMA, H
ITO, H
SHIMOSATO, T
TAHARA, E
机构
[1] HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,1-2-3 KASUMI,MINAMI KU,HIROSHIMA 734,JAPAN
[2] HIROSHIMA UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG 2,MINAMI KU,HIROSHIMA 734,JAPAN
关键词
D O I
10.1038/bjc.1990.265
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To determine whether alterations in oncogenes are associated with tumour progression and metastasis, DNAs from 32 metastatic tumour samples of different sites in 12 autopsy cases of gastric carcinomas were analysed for alterations of ERBB, ERBB2, HST1, INT2 and LMYC genes by Southern blot hybridisation. DNAs from 89 primary gastric carcinomas including 69 advanced carcinomas and 20 early carcinomas were also examined. In primary tumours, no amplification was detected in early carcinomas, while amplification of ERBB and ERBB2 genes was detected in one (1.4%) and four (5.8%) out of 69 advanced carcinomas, respectively. In metastatic tumours, amplification of ERBB gene was detected in three metastatic tumours (9.4%), and all of them had allelic deletion of the LMYC gene. Regardless of histological type, amplification of ERBB2 gene was detected in 8 metastatic tumours (25.0%), out of which three tumours had coamplification of HST1 and INT2 genes. The incidence of ERBB2 amplification in metastatic tumours was significantly higher than that in primary tumours. These results indicate that multi-alterations in oncogenes might occur during tumour progression and metastasis of human gastric carcinomas. © The MacMillan Press Ltd., 1990.
引用
收藏
页码:226 / 230
页数:5
相关论文
共 35 条
[1]  
ALITALO K, 1987, ONCOGENES GROWTH FAC, P17
[2]   CHROMOSOME-17 DELETIONS AND P53 GENE-MUTATIONS IN COLORECTAL CARCINOMAS [J].
BAKER, SJ ;
FEARON, ER ;
NIGRO, JM ;
HAMILTON, SR ;
PREISINGER, AC ;
JESSUP, JM ;
VANTUINEN, P ;
LEDBETTER, DH ;
BARKER, DF ;
NAKAMURA, Y ;
WHITE, R ;
VOGELSTEIN, B .
SCIENCE, 1989, 244 (4901) :217-221
[3]   RAS GENES [J].
BARBACID, M .
ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :779-827
[4]  
DUBEAU L, 1986, CANCER RES, V46, P2964
[5]   CLONAL ALLELE LOSS IN GASTROINTESTINAL CANCERS [J].
FEY, MF ;
HESKETH, C ;
WAINSCOAT, JS ;
GENDLER, S ;
THEIN, SL .
BRITISH JOURNAL OF CANCER, 1989, 59 (05) :750-754
[6]   DETECTION OF HIGH-INCIDENCE OF K-RAS ONCOGENES DURING HUMAN-COLON TUMORIGENESIS [J].
FORRESTER, K ;
ALMOGUERA, C ;
HAN, KY ;
GRIZZLE, WE ;
PERUCHO, M .
NATURE, 1987, 327 (6120) :298-303
[7]   PURIFICATION OF DNA FROM FORMALDEHYDE FIXED AND PARAFFIN EMBEDDED HUMAN-TISSUE [J].
GOELZ, SE ;
HAMILTON, SR ;
VOGELSTEIN, B .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 130 (01) :118-126
[8]   RECESSIVE MECHANISMS OF MALIGNANCY [J].
GREEN, AR .
BRITISH JOURNAL OF CANCER, 1988, 58 (02) :115-121
[9]  
JOHNSON BE, 1988, CANCER RES, V48, P5163
[10]   CELLULAR ONCOGENES AND MULTISTEP CARCINOGENESIS [J].
LAND, H ;
PARADA, LF ;
WEINBERG, RA .
SCIENCE, 1983, 222 (4625) :771-778