IMPROVEMENT OF THE PRODUCTION OF 5-METHOXYPODOPHYLLOTOXIN USING A NEW SELECTED ROOT CULTURE OF LINUM-FLAVUM L

被引:21
作者
VANUDEN, W
PRAS, N
HOMAN, B
MALINGRE, TM
机构
[1] Laboratory for Pharmacognosy, University of Groningen, Groningen, NL-9713 AW
关键词
LINUM-FLAVUM; 5-METHOXYPODOPHYLLOTOXIN; OPTIMIZATION; PHENYLPROPANOIDS; PRODUCTION; ROOT CULTURE;
D O I
10.1007/BF00041279
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A new, morphologically root-like cell line of Linum flavum was selected and used for the optimization of the production of 5-methoxypodophyllotoxin. The omittance of naphthaleneacetic acid from the medium resulted in further root formation, which was accompained with a 2.6 fold increase of 5-methoxypodophyllotoxin levels. The feeding of the phenylpropanoids L-tyrosine and L-phenylalanine resulted in enhanced 5-methoxypodophyllotoxin contents. After the removal of vitamins together with the phytohormone from the medium, 5-methoxypodophyllotoxin levels were enhanced 6 times. The maximal content ever found using this production medium was 1.01% on a dry weight basis. The application of higher sucrose concentrations led to proportional biomass increase, cells grown on 6% sucrose resulted in the highest yield, 121.4 mg 1(-1) 5-methoxypodophyllotoxin.
引用
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页码:115 / 121
页数:7
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