CLONING AND CHARACTERIZATION OF A GLUTAMATE TRANSPORTER CDNA FROM HUMAN CEREBELLUM

被引：57

作者：

SHASHIDHARAN, P ^{[1
]}

PLAITAKIS, A ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT NEUROL,1 GUSTAVE L LEVY PL,NEW YORK,NY 10029

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1216卷 / 01期

关键词：

GLUTAMATE TRANSPORTER; CDNA; CEREBELLUM; CLONING; SEQUENCE; (HUMAN);

D O I：

10.1016/0167-4781(93)90057-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glutamate/aspartate transporters are essential for the elimination and recycling of synaptic glutamate released from nerve endings during neurotransmission. Evidence suggests that these processes are altered in ischemia and neuronal degenerations linked to excitotoxicity. We screened a cDNA library constructed from human cerebellar mRNA, and isolated a cDNA that shows an 88.5% and a 98.7% sequence similarity at the nucleotide and amino acid level, respectively, with a rat brain specific Na+-dependent glutamate/aspartate transporter. The human cDNA is expressed in brain and it may prove useful in the study of human neurodegenerations linked to glutamate dysfunction.

引用

页码：161 / 164

页数：4

共 14 条

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