MODULATION OF DOPAMINE-INDUCED CAMP PRODUCTION IN RAT STRIATAL CULTURES BY THE CALCIUM IONOPHORE A23187 AND BY PHORBOL-12-MYRISTATE-13-ACETATE

被引：10

作者：

SCHINELLI, S ^{[1
]}

PAOLILLO, M ^{[1
]}

CORONA, GL ^{[1
]}

机构：

[1] UNIV PAVIA,FAC FARM,IST FARMACOL,I-27100 PAVIA,ITALY

来源：

MOLECULAR BRAIN RESEARCH | 1994年 / 21卷 / 1-2期

关键词：

STRIATAL NEURON; ADENYLATE CYCLASE; DOPAMINE; CALCIUM IONOPHORE A23187; PROTEIN KINASE C; PHORBOL MYRISTATE ACETATE;

D O I：

10.1016/0169-328X(94)90389-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The modulation of cAMP formation by protein kinase C (PKC), activated by phorbol-12-myristate-13-acetate, and by Ca2+ entry, using the ionophore A23187, was investigated in rat striatal neurons grown in primary dissociated cell culture. Phorbol-12-myristate-13-acetate (PMA) potentiated forskolin-induced and dopamine-induced cAMP formation in a concentration-dependent manner. In contrast, the calcium ionophore A23187 inhibited dopamine-induced cAMP formation. When PMA and A23187 were tested simultaneously, the levels of cAMP were not statistically different from those found in the presence of dopamine alone. Furthermore, the decreasing effect of A23187 on cAMP formation was enhanced when PKC was desensitized by pretreating the neurons with 1 mu M PMA for 18 h. These data indicate that in striatal neurons Ca2+ entry and PKC activation exert opposing effect on cAMP production.

引用

页码：162 / 166

页数：5

共 29 条

[1] PROTEIN-KINASE-C, CALCIUM AND PHOSPHOLIPID DEGRADATION [J].