C-MYC PROTEIN EXPRESSION DURING CELL-CYCLE PHASES IN DIFFERENTIATING HL-60 CELLS

被引：10

作者：

KUMAKURA, S

ISHIKURA, H

TSUMURA, H

HAYASHI, H

ENDO, J

TSUNEMATSU, T

机构：

[1] Third Division of Internal Medicine and Division of Blood Transfusion, Shimane Medical University

来源：

LEUKEMIA & LYMPHOMA | 1994年 / 14卷 / 1-2期

关键词：

C-MYC PROTEIN; CELL CYCLE; FLOW CYTOMETRY; HL-60; CELL; DMSO; DIFFERENTIATION;

D O I：

10.3109/10428199409049665

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We examined c-myc protein expression in cell cycle phases during differentiation induction of HL-60 cells by flow cytometry using an indirect immunofluorescence method. In exponentially proliferating HL-60 cells, c-myc protein was expressed in a cell cycle dependent manner. During the differentiation induction of HL-60 cells with dimethylsulfoxide (DMSO), c-myc protein was rapidly down-regulated in the G(1/0) specific phase prior to the appearance of differentiation associated markers. Our results indicate that c-myc protein functions in the G(1/0) specific phase in cellular differentiation, and the rapid down-regulation of c-myc protein in G(1/0) phase is closely associated with initial differentiation programs.

引用

页码：171 / 180

页数：10

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