TRANSDERMAL TRANSPORT OF DNA ANTISENSE OLIGONUCLEOTIDES BY ELECTROPORATION

被引：80

作者：

ZEWERT, TE ^{[1
]}

PLIQUETT, UF ^{[1
]}

LANGER, R ^{[1
]}

WEAVER, JC ^{[1
]}

机构：

[1] HARVARD UNIV,MIT,DIV HLTH SCI & TECHNOL,CAMBRIDGE,MA 02139

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1968

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescein-labeled antisense oligodeoxynucleotides (ODNs) corresponding to the promoters of the protooncogene c-myb (24-mer) and the oncogene c-myc (15-mer) were transported through the human skin in vitro by electroporation. Fluxes of 6.4+/-2.1 pM/cm(2)* hr and 11.5+/-3.5 pM/cm(2)* hr, respectively, were achieved during exponential pulsing [tau(pulse) = 1.1ms, transdermal voltage (U-skin)= 80V] every five seconds. The flux for U-skin <70V was undetectable, rapidly increased at 80V, but plateaued at higher values. Fluorescence imaging demonstrated that transport of the ODNs is concentrated in localized transport regions (LTRs)(1) approximately 30 mu m in diameter. (C) 1995 Academic Press, Inc.

引用

页码：286 / 292

页数：7

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