POTENT INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION BY AN INTRACELLULAR ANTI-REV SINGLE-CHAIN ANTIBODY

被引：122

作者：

DUAN, LX ^{[1
]}

BAGASRA, O ^{[1
]}

LAUGHLIN, MA ^{[1
]}

OAKES, JW ^{[1
]}

POMERANTZ, RJ ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT MED,DIV INFECT DIS,MOLEC RETROVIROL SECT,PHILADELPHIA,PA 19107

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 11期

关键词：

D O I：

10.1073/pnas.91.11.5075

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human immunodeficiency virus type 1 (HIV-1) has a complex life cycle, which has made it a difficult target for conventional therapeutic modalities. A single-chain antibody moiety, directed against the HIV-1 regulatory protein Rev, which rescues unspliced viral RNA from the nucleus of infected cells, has now been developed. This anti-Rev single-chain construct (SFv) consists of both light and heavy chain variable regions of an anti-Rev monoclonal antibody, which, when expressed intracellularly within human cells, potently inhibits HIV-1 replication. This intracellular SFv molecule is demonstrated to specifically antagonize Rev function. Thus, intracellular SFv expression, against a retroviral regulatory protein, may be useful as a gene therapeutic approach to combat HIV-1 infections.

引用

页码：5075 / 5079

页数：5

共 21 条

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