EVALUATION OF MOBILE-PHASE ADDITIVES IN LC-SYSTEMS USING CHEMOMETRICS

A strategy for general optimization of the mobile phase in LC-systems is described in this study. The influence on column efficiencies and capacity factors of three uncharged modifiers, two charged modifiers, pH and column temperature was evaluated with experimental design and multivariate analyses. A factorial design was used and the experimental results demonstrated that the uncharged modifiers and the pH were important for the capacity factors while column temperature and pH were important for the column efficiency. Two different stationary phase materials were used and compared, one was a traditional silica based material and the other a silica covered with a polymer. Both were reversed phase materials. A partial least squares model obtained from the results from the polymer column, Chromspher B, was used to predict retention data.